Journal
COMPOSITES PART B-ENGINEERING
Volume 222, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.compositesb.2021.109057
Keywords
Photocrosslinked hydrogel; Phosphate functionalized chitosan; Bioceramic nanoparticles; Osteogenesis; Angiogenesis
Funding
- National & Local Joint Engineering Research Center of Orthopaedic Biomaterials [XMHT20190204007]
- Natural Science Foundation of China [82001319]
- National Science Foundation of Guangdong Province [2018A030310670, 2019A1515011290]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515110983]
- San Ming Project of Medicine in Shenzhen [SZSM201612092]
- Research and Development Projects of Shenzhen [JCYJ20170307111755218]
- Bethune Charitable Foundation
- CSPC Osteoporosis Research Foundation Project [G-X-2020-1107-21]
- Science and Technology Innovation Commission of Shenzhen Municipality [JCYJ20170818163724754]
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This study developed a new water-soluble phosphate functionalized chitosan and fabricated a CSMAP-SPS composite hydrogel, demonstrating excellent biomineralization activity and biomedical potential.
The natural polysaccharide-based hydrogels have gained significant interest to use as 3D scaffolds for bone repair and reconstruction owing to their excellent biocompatibility, biosafety, and biodegradability. Yet, these hydrogels have major obstacles in terms of insufficient properties such as mechanical, osteogenic, angiogenic, and biomineralization. Therefore, this study mainly aims to develop an innovative water-soluble phosphate functionalized chitosan (CSMAP) through consequent modification with methacrylic anhydride (MA), then alteration by phosphonopropionic acid (P). Further, a novel photocrosslinked composite hydrogel was fabricated with a combination of CSMAP and synthesized strontium phosphosilicate (SPS, Sr5(PO4)2SiO4) bioceramic nanoparticles with a photoinitiator under UV irradiation. The various concentrations of nanosized SPS particles (0.5, 2.5, 5, and 10 mg/mL) were incorporated into the CSMAP matrix and the swelling, mechanical, morphological, physiochemical, osteogenic, angiogenic properties were investigated. The CSMAP-SPS composite hydrogels possessed a well-arranged porous network structure. The composite hydrogel was restricted to swell in distilled water with the increased concentration of SPS particles. The compressive strength and modulus of CSMAP hydrogel were improved by the inclusion of SPS particles. The bioactive Sr, P, and Si ions were released from CSMAP-SPS hydrogels in a sustained and controlled manner at a non-toxic level. The composite hydrogels promoted in vitro amorphous apatite deposition, revealing a superior biomineralization activity. In vitro results demonstrated that the composites hydrogels showed no toxicity to preosteoblast MC3T3-E1 cells, induced MC3T3-E1 osteogenic differentiation with detection of bsp, ocn and opn osteogenic genes, and also promoted the endothelial tube formation with increased capillary length, branch points, and angiogenic gene vegf expression. Thus, the obtained results proved that the fabricated novel CSMAP-SPS hydrogel could act as an attractive candidate for bone regeneration.
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