Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
Volume 40, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbd.2021.100882
Keywords
Grapholita molesta; G protein-coupled receptors; Neuropeptides; Peptidomics; Transcriptomics; Tissue expression pattern
Funding
- China Agriculture Research System of MOF and MARA
Ask authors/readers for more resources
A study was conducted to analyze the CNS of the oriental fruit moth, identifying numerous neuropeptide precursor genes, mature peptides, and neuropeptide GPCR genes. The study also explored the expression patterns of these genes in larval tissues. These findings will aid in further investigations on the physiological functions and pharmacological characterization of neuropeptides and their GPCRs in the oriental fruit moth, as well as the development of specific neuropeptide-based agents for pest control.
The oriental fruit moth Grapholita molesta is a cosmopolitan pest of orchard, which causes serious economic losses to the fruit production. Neuropeptides and their specific receptors (primarily G protein-coupled receptors, GPCRs) regulate multiple biological functions in insects and represent promising next-generation pest management strategy. Here, we generated a transcriptome of the central nervous system (CNS) of G. molesta. Overall, 57 neuropeptide precursor genes were identified and 128 various mature peptides were predicted from these precursors. Using peptidomic analysis of CNS of G. molesta, we identified total of 28 mature peptides and precursor-related peptides from 16 precursors. A total of 41 neuropeptide GPCR genes belonging to three classes were also identified. These GPCRs and their probable ligands were predicted. Additionally, expression patterns of these 98 genes in various larval tissues were evaluated using quantitative real-time PCR. Taken together, these results will benefit further investigations to determine physiological functions and pharmacological characterization of neuropeptides and their GPCRs in G. molesta; and to develop specific neuropeptide-based agents for this tortricid fruit pest control.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available