The cadmium toxicity in gills of Mytilus coruscus was accentuated by benzo (a)pyrene of higher dose but not lower dose

In natural environment, the existence of interactions of toxic mixtures could induce diverse biochemical pathways and consequently exert different toxicological responses in aquatic organisms. However, little information is available on the effects of combined xenobiotics on lower aquatic invertebrates. Here, we assessed the effects of cadmium (Cd, 0.31 mg/L) as well as the mixture of Cd (0.31 mg/L) and benzo(a)pyrene (Bap, 5 or 50 mu g/L) on bioaccumulation, antioxidant, lipid peroxidation (LPO) and metallothionein (MT) responses in gills of thick shell mussel Mytilus coruscus. Upon exposed to single Cd, the metal bioaccumulation, antioxidant enzymes activities, LPO and MT level significantly increased in the gills, suggesting an apparent toxicity to mussels. The interaction of Cd + 5 mu g/L Bap did not significantly alter these endpoints compared to single Cd. However, once the dose of Bap elevated to 50 mu g/L, the induction of bioaccumulation, antioxidant system and LPO was even more pronounced while the induction of MT was remarkably inhibited, implying an accentuated toxicity. Collectively, the current results demonstrated that 0.31 mg/L Cd exposure resulted in severe toxicity to mussels despite of the induction of MT system to alleviate the metal toxicity. Once the Cd exposure combined with Bap, the lower dose of Bap could not change the Cd toxicity while the higher dose of Bap accentuated the toxicity by inhibiting metallothionein synthesis. These findings might provide some useful clues for elucidation the mechanism of the interaction of combined xenobiotics in molluscs.

Automated summary

In a natural environment, interactions of toxic mixtures may lead to diverse biochemical pathways and toxicological responses in aquatic organisms. The study found that exposure to Cd resulted in significant toxicity to mussels, and the addition of Bap at a higher dose accentuated the toxicity by inhibiting metallothionein synthesis. These findings offer insights into the mechanisms of combined xenobiotics in molluscs.