Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 213, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2022.112420
Keywords
Anderson-Evans polyoxometalate; Metal-organic framework; Cytochrome C; Separation and purification; Solid phase extraction
Funding
- Natural Science Foundation of China [21804093, 31900366, 82003693]
- PhD Start-up Foundation of Liaoning Province [2020-BS-266, 2021-BS-284]
- Science Research Foundation of Education Department for Liaoning Province
Ask authors/readers for more resources
In this study, a novel Anderson-Evans polyoxometalate-based metal-organic framework composite TeW6 @ZIF-8 was successfully synthesized and characterized. The results showed that the composite possessed selective adsorption ability towards cytochrome C, as well as good stability and biocompatibility. Furthermore, a new strategy for separating and purifying cytochrome C from porcine heart using TeW6 @ZIF-8 composite as an adsorbent was established.
Anderson-Evans type polyoxometalate group (Na-6[TeW6O24].22 H2O, TeW6) was combined with porous metal organic framework ZIF-8 by electrostatic interaction to obtain a novel Anderson-Evans polyoxometalate-based metal-organic framework composite, TeW6 @ZIF-8. FT-IR, Raman, XRD, TG, DSC, SEM, and TEM were used to characterize the composite. It was proved that the Anderson-Evans type polyoxometalate group TeW6 was successfully hybridized with metal-organic framework ZIF-8, and the composite possesses good stability. Based on the potential interaction between TeW6 and proteins and the coordination between imidazole groups in ZIF-8 and proteins with a porphyrin ring structure, the adsorption selectivity towards different proteins on the TeW6 @ZIF-8 composite was studied in this work. The experiment results showed that the TeW6 @ZIF-8 composite was selectively adsorbed to cytochrome C. At pH 11.0, the adsorption efficiency of 94.01% was obtained for processing 1.0 mL 100 mu g mL(-1) cytochrome C with 3.0 mg TeW6 @ZIF-8 composite. The adsorption behavior of cytochrome C fits well with the Langmuir adsorption model, corresponding to a theoretical adsorption capacity of 232.56 mg g(-1). The retained cytochrome C could be readily recovered by 1% SDS (m/m), giving rise to a recovery of 65.6%. Circular dichroism spectra indicate no conformational change for cytochrome C after the adsorption and desorption processes, demonstrating the favorable biocompatibility of TeW6 @ZIF-8 composite. In applying practical samples, SDS-PAGE results showed that cytochrome C was successfully isolated and purified by TeW6 @ZIF-8 composite from porcine heart protein extract, which is further identified with LC-MS/MS. Thus, a new strategy for separating and purifying cytochrome C from the porcine heart using TeW6 @ZIF-8 composite as an adsorbent was established.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available