Increased concentrations of myeloperoxidase in serum and serum extracellular vesicles are associated with type 2 diabetes mellitus

Background: Inflammatory response plays a critical role in the initiation and progression of type 2 diabetes mellitus (T2DM). Myeloperoxidase (MPO), a leukocyte-derived protagonist, exerts its proinflammatory properties in many complications. We explored the associations between serum extracellular vesicle (EV)-derived MPO as well as serum MPO and T2DM. Methods: We performed a cross-sectional study in 151 individuals, including 93 patients with T2DM and 58 nonT2DM controls. The concentrations of serum EV-derived MPO and serum MPO were measured by Luminex Assay. Results: Our data showed that serum EV-derived MPO concentrations and serum MPO concentrations were significantly higher in T2DM patients compared with non-T2DM subjects. In addition, multivariate logistic regression analysis revealed that serum EV-derived MPO as well as serum MPO was independently associated with the presence of T2DM even after adjusting for confounding factors (OR = 1.836 /1 ng EV-derived MPO, 95% CI = 1.395-2.417, P < 0.001; OR = 4.135 /10 ng serum MPO, 95% CI = 2.285-7.483, P < 0.001). Furthermore, serum MPO showed marginally higher discriminatory accuracy than serum EV-derived MPO in screening T2DM (AUC = 0.858; AUC = 0.779). Conclusion: Increased concentrations of the inflammatory marker MPO either in serum or in serum EVs were independently associated with T2DM.

Automated summary

The study found that the concentrations of serum EV-derived MPO and serum MPO were significantly higher in T2DM patients compared with non-T2DM subjects. Multivariate logistic regression analysis showed that both serum EV-derived MPO and serum MPO were independently associated with the presence of T2DM even after adjusting for confounding factors.