4.5 Article

Attenuation of Oxidative Stress-Induced Cell Apoptosis and Pyroptosis in RSC96 Cells by Salvianolic Acid B

Journal

CHINESE JOURNAL OF INTEGRATIVE MEDICINE
Volume 28, Issue 3, Pages 243-248

Publisher

SPRINGER
DOI: 10.1007/s11655-021-3507-2

Keywords

apoptosis; pyroptosis; salvianolic acid B; hyperglycemia

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The study showed that Sal B can protect against diabetic peripheral neuropathy by attenuating apoptosis and pyroptosis. Results demonstrated that Sal B could inhibit high glucose-induced damage, including reduced cell proliferation and oxidative stress.
Objective To determine whether salvianolic acid B (Sal B) exerts protective effects on diabetic peripheral neuropathy by attenuating apoptosis and pyroptosis. Methods RSC96 cells were primarily cultured with DMEM (5.6 mmol/L glucose), hyperglycemia (HG, 125 mmol/L glucose) and Sal B (0.1, 1, and 10 mu mol/L). Cells proliferation was measured by 3-(4, 5-cimethylthiazol-2-yl)-2, 5-dilphenyltetrazolium bromide assay. Reactive oxygen species (ROS) generation and apoptosis rate were detected by flow cytometry analysis. Western blot was performed to analyze the expressions of poly ADP-ribose polymerase (PARP), cleaved-caspase 3, cleaved-caspase 9, Bcl-2, Bax, NLRP3, ASC, and interleukin (IL)-1 beta. Results Treatment with HG at a concentration of 125 mmol/L attenuated cellular proliferation, while Sal B alleviated this injury (P<0.05). In addition, Sal B inhibited HG-induced ROS production and apoptosis rate (P<0.05). Furthermore, treatment with Sal B down-regulated HG-induced PARP, cleaved-caspase 3, cleaved-caspase 9, Bax, NLRP3, ASC, and IL-1 beta expression, but mitigated HG-mediated down-regulation of Bcl-2 expression (P<0.05). Conclusion Sal B may protect RSC96 cells against HG-induced cellular injury via the inhibition of apoptosis and pyroptosis activated by ROS.

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