Journal
CHINESE CHEMICAL LETTERS
Volume 33, Issue 4, Pages 1913-1916Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2021.11.025
Keywords
Aptamer; Colorimetric aptasensor; SELEX; Chlorpyrifos; AuNPs
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The authors developed a simple gold nanoparticles-based colorimetric biosensor for chlorpyrifos detection, utilizing an aptamer as the capture probe. The method exhibited excellent sensitivity and reliable detection performance, making it a promising tool for chlorpyrifos detection in water and agricultural products.
In this work, a simple gold nanoparticles (AuNPs) based colorimetric biosensor was developed for chlorpyrifos (Chl) detection using an aptamer as the capture probe. The Chl-aptamer with low dissociation constant (K-d) of 58.59 +/- 6.08 nmol/L was selected by ssDNA library immobilized systematic evolution of ligands by enrichment (SELEX). In the absence of Chl, the Chl-aptamer acted as the stabilizer for AuNPs in salt solution. In the presence of Chl, the highly specific Chl-aptamer bound with Chl targets immediately, thus a self-aggregation of AuNPs induced by salt was displayed. The fabricated colorimetric aptasensor exhibited an excellent sensitivity for Chl detection with the LOD as low as 14.46 nmol/L. In addition, the aptasensor was applied to test Chl in tap water, cucumber and cabbage samples, the excellent recoveries with acceptable RSD values below 5% demonstrated that the method can be considered as a promising tool for simple, rapid Chl detection. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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