4.7 Article

Multiple exosome RNA analysis methods for lung cancer diagnosis through integrated on-chip microfluidic system

Related references

Note: Only part of the references are listed.
Review Chemistry, Analytical

Microfluidics for Liquid Biopsies: Recent Advances, Current Challenges, and Future Directions

Yuri Belotti et al.

ANALYTICAL CHEMISTRY (2021)

Article Chemistry, Medicinal

Effective degradation of EGFRL858R+T790M mutant proteins by CRBN-based PROTACs through both proteosome and autophagy/lysosome degradation systems

Xiaojuan Qu et al.

Summary: PROTAC technology offers a promising strategy to target EGFR protein and overcome drug resistance in non-small cell lung cancer patients with EGFR mutations. The involvement of autophagy/lysosome system in PROTAC-mediated target protein degradation is demonstrated in this study, providing new insights into cancer treatment.

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2021)

Review Genetics & Heredity

Liquid Biopsies: Applications for Cancer Diagnosis and Monitoring

Ivana Martins et al.

Summary: Liquid biopsies are a revolutionary approach for cancer management as they can provide a more comprehensive characterization of tumor profiles through the detection of circulating tumor-derived components in biofluids. They have the potential to assist in diagnosis, prognosis, treatment selection, as well as monitoring disease evolution and treatment response in real-time. Despite their value in oncology, some limitations still need to be addressed for their routine clinical implementation.

GENES (2021)

Review Medicine, Research & Experimental

qPCR and qRT-PCR analysis: Regulatory points to consider when conducting biodistribution and vector shedding studies

Haiyan Ma et al.

Summary: The growth of gene and cell therapy fields has led to increased demand for safety assessments, resulting in the need for regulated biodistribution, vector shedding, gene expression, and pharmacokinetics studies. While guidance documents recommend qPCR and qRT-PCR for detection, criteria such as accuracy and precision are not yet defined, leading to conflicting interpretations and an urgent need for consensus among bioanalytical scientists.

MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT (2021)

Article Biophysics

Ultrafast multiplexed detection of SARS-CoV-2 RNA using a rapid droplet digital PCR system

Hao Yin et al.

Summary: The rapid digital PCR system combines droplet digital and rapid PCR techniques for efficient, accurate, and quantitative detection of SARS-CoV-2 RNA. Benchmark tests with serial diluted reference samples demonstrate the high accuracy of the system, while qPCR may yield significant variations in Ct values leading to false-negative results.

BIOSENSORS & BIOELECTRONICS (2021)

Review Biochemistry & Molecular Biology

Digital PCR for accurate quantification of pathogens: Principles, applications, challenges and future prospects

Shuwen Lei et al.

Summary: Digital PCR (dPCR) has gained considerable attention for its ability to quantitate different types of pathogens with desired accuracy, sensitivity, and reproducibility, using end-point measurement for absolute quantification without the need for standard curves. It provides guidance for further genetic analysis and molecular diagnosis, contributing to the quantification of low level of nucleic acid and early detection of pathogenic diseases. The benefits, challenges, and future prospects of dPCR have been highlighted in this review, showcasing its potential as a powerful tool for pathogen detection and control.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Review Medicine, General & Internal

Clinical Applications of Liquid Biopsy in Non-Small Cell Lung Cancer Patients: Current Status and Recent Advances in Clinical Practice

Shinhee Park et al.

Summary: Liquid biopsy, focusing on analysis of tumor-derived material in blood, particularly cell-free tumor DNA in plasma, has become a valuable tool in understanding genomic alterations in cancer evolution, especially in non-small cell lung cancer for guiding personalized targeted therapies.

JOURNAL OF CLINICAL MEDICINE (2021)

Review Multidisciplinary Sciences

The biology, function, and biomedical applications of exosomes

Raghu Kalluri et al.

SCIENCE (2020)

Review Biochemistry & Molecular Biology

Application of exosomes as liquid biopsy in clinical diagnosis

Biting Zhou et al.

SIGNAL TRANSDUCTION AND TARGETED THERAPY (2020)

Review Cell Biology

Exosomal miRNAs as novel cancer biomarkers: Challenges and opportunities

Mahsa Salehi et al.

JOURNAL OF CELLULAR PHYSIOLOGY (2018)

Review Cell Biology

RNA in extracellular vesicles

Kyoung Mi Kim et al.

WILEY INTERDISCIPLINARY REVIEWS-RNA (2017)

Review Chemistry, Multidisciplinary

Emerging Droplet Microfluidics

Luoran Shang et al.

CHEMICAL REVIEWS (2017)

Article Oncology

Cancer Statistics in China, 2015

Wanqing Chen et al.

CA-A CANCER JOURNAL FOR CLINICIANS (2016)

Article Oncology

KRAS Mutations in Lung Cancer

Niki Karachaliou et al.

CLINICAL LUNG CANCER (2013)

Article Respiratory System

Pathology of Lung Cancer

William D. Travis

CLINICS IN CHEST MEDICINE (2011)

Review Cell Biology

Shedding microvesicles: artefacts no more

Emanuele Cocucci et al.

TRENDS IN CELL BIOLOGY (2009)

Review Immunology

Exosomes: From biogenesis and secretion to biological function

Sascha Keller et al.

IMMUNOLOGY LETTERS (2006)

Article Biochemical Research Methods

Quantification of mRNA using real-time RT-PCR

Tania Nolan et al.

NATURE PROTOCOLS (2006)

Review Cell Biology

The biogenesis and functions of exosomes

W Stoorvogel et al.

TRAFFIC (2002)