Journal
CHEMSUSCHEM
Volume 15, Issue 9, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cssc.202102152
Keywords
10-hydroxy-2-decenoic acid; biocatalysis; enzyme catalysis; metabolic engineering; unsaturated carboxylic acids
Funding
- Science Foundation of ShanDong Province [ZR2019MC010, ZR2017ZB0208]
- Synthetic Biology Technology Innovation Center of Shandong Province [sdsynbio-2018-PY-02]
- Foundation of Qilu University of Technology of Cultivating Subject for Biology and Biochemistry [202014]
- National Science Foundation of China [31801527]
- Focus on Research and Development Plan in Shandong Province [2019JZZY011003, 2020CXGC010603]
- National Key Research and Development Project [2019YFC1905900]
- Taishan industry leading talent [tscy20180103]
Ask authors/readers for more resources
The study modified the beta-oxidation pathway of Escherichia coli and designed a P450 terminal hydroxylase (CYP153A33-CPRBM3) for the synthesis of 10-HDA, achieving a yield of 217 mg L-1 from decanoic acid.
10-Hydroxy-2-decenoic acid (10-HDA) is a terminal hydroxylated medium-chain alpha,beta-unsaturated carboxylic acid that performs various unique physiological activities and has a wide market value. Therefore, development of an environmentally friendly, safe, and high-efficiency route to synthesize 10-HDA is required. Here, the beta-oxidation pathway of Escherichia coli was modified and a P450 terminal hydroxylase (CYP153A33-CPRBM3) was rationally designed to synthesize 10-HDA using decanoic acid as a substrate via two-step whole-cell catalysis. Different homologues of FadDs, FadEs, and YdiIs were analyzed in the first step of the conversion of decanoic acid to trans- -2- decenoic acid. In the second step, CYP153A33 (M228L)-CPRBM3 efficiently catalyzed the conversion of trans- -2- decenoic acid to 10-HDA. Finally, 217 mg L-1 10-HDA was obtained with 500 mg L-1 decanoic acid. This study provides a strategy for biosynthesis of 10-HDA and other alpha, beta-unsaturated carboxylic acid derivatives from specific fatty acids.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available