Exploration and verification of the feasibility of the sulfur-based autotrophic denitrification integrated biomass-based heterotrophic denitrification systems for wastewater treatment: From feasibility to application

The sulfur-based autotrophic denitrification (SAD) and the solid organic carbon-based denitrification processes are both efficient techniques to remove nitrate from wastewater, and the hydrogen ions generated by the SAD process would be consumed in the heterotrophic denitrification process. Therefore, it is possible to improve the denitrification capacity when the solid organic carbon was added into a SAD reactor. In this study, corncob powder and sawdust powder were selected as solid organic carbon sources, and the sulfur-based autotrophic denitrification integrated biomass-based heterotrophic denitrification system was formed (SBD). The laboratory and field experiments showed that SBD could shorten the start -up period, decrease the sulfate productivity, and maintain a good denitrification performance when treated wastewater. According to the field experiment results, when the HRT was 1 h, the effluent total nitrogen (TN) concentration was always lower than 15 mg L-1. In addition, nitrite inhibition was observed when the concentration of nitrite in the reactors reached above 30 mg L-1. The mixture of elemental sulfur powder, shell powder, corncob powder, and sawdust powder with a mass ratio of 6:2:1:1 was the optimal filter for the SBD system, with an average nitrate reduction rate (NAR) of 420 mg NO3-N.L-1.d(-1) obtained at the end of the study. During the whole operation, the major autotrophs in the SBD systems were Thermomonas, Ferritrophicum, and Thiobacillus, while the major heterotrophs were Saprospiraceae, Ferruginibacter, Dokdonella, and Simplicispira. Overall, the SBD system was a feasible and practically favorable way to remove nitrate from wastewater.

Automated summary

The study demonstrated that the sulfur-based autotrophic denitrification integrated with biomass-based heterotrophic denitrification system (SBD) is an efficient way to remove nitrate from wastewater, showing advantages such as shortening start-up period, reducing sulfate productivity, and maintaining good denitrification performance. The optimal filter for the SBD system was a mixture of elemental sulfur powder, shell powder, corncob powder, and sawdust powder, with an average nitrate reduction rate of 420 mg NO3-N.L-1.d(-1) achieved at the end of the study. Major autotrophs in the SBD systems included Thermomonas, Ferritrophicum, and Thiobacillus, while major heterotrophs included Saprospiraceae, Ferruginibacter, Dokdonella, and Simplicispira, indicating the feasibility and practicality of the SBD system for nitrate removal from wastewater.