Exposures to deltamethrin on immature Chironomus columbiensis drive sublethal and transgenerational effects on their reproduction and wing morphology

Sublethal exposure to insecticides can trigger unintended responses in non-target insects that may disrupt reproductive and developmental performances of these organisms. Here, we assessed whether sublethal exposure to the pyrethroid insecticide deltamethrin in early life had sublethal and transgenerational effects on the reproduction (i.e., fecundity and fertility) and wing morphology of Chironomus columbiensis, an aquatic insect used as a water quality indicator. We first conducted concentration-response bioassays to evaluate the susceptibility of C. columbiensis larvae to deltamethrin. Our results revealed that deltamethrin toxicity was approximately 7-fold higher when C. columbiensis larvae where exposed to 96 h (LC50 = 0.17 [0.15-0.20] mu g/L) than to 24 h (LC50 = 1.17 [0.97-1.43] mu g/L). Furthermore, the sublethal exposures (at LC1 = 0.02 mu g/L or LC50 = 0.05 mu g/L) of immature C. columbiensis resulted in lower fecundity (e.g., reduced eggs production) and morphometric variation wing shapes. Further reduction in fertility rates (quantity of viable eggs) occurred at deltamethrin LC10 (0.05 mu g/L). Almost 80% of the fecundity was recovered with only a single recovery generation; however, two subsequent recovery generations were not sufficient to fully recover fecundity in C. columbiensis. Specimens recovered from 98.5% of wing morphometric variation after two consecutive generations without deltamethrin exposure. Collectively, our findings demonstrates that sublethal exposure to synthetic pyrethroids such as deltamethrin detrimentally affect the reproduction and wing shape of C. columbiensis, but also indicate that proper management of these compounds (e.g., concentration and frequency of application) would suffice for these insects' population recovery.

Automated summary

Sublethal exposure to the pyrethroid insecticide deltamethrin in early life has been shown to have negative effects on the reproduction and wing morphology of the aquatic insect Chironomus columbiensis. The study found that sublethal exposures resulted in reduced fecundity and morphometric wing shape variations. Proper management of these compounds could aid in the recovery of population.