Efficient biostimulants for bacterial quorum quenching to control fouling in MBR

Quorum quenching (QQ), which disrupts bacterial communication and biofilm formation, could alleviate biofouling in MBR. QQ bio-stimulus possessing similar conserved moiety as the signal molecule could promote indigenous QQ bacteria, and thus successfully alleviate biofouling in MBR. However, efficient biostimulant has been barely explored for QQ enhancement in activated sludge system. This study extensively enumerated the potential QQ bio-stimuli, and examined their efficacy on QQ promotion for activated sludge. Moreover, the effect of the QQ consortia on fouling mitigation was also investigated. The results indicated that gamma-caprolactone (GCL), D-xylonic acid-1,4-lactone (XAL), gamma-heptalactone (GHL), urea, and acetamide proved effective in promoting AHLs inactivating activity of activated sludge. GCL, XAL, and GHL intensified the lactonase activity, while urea and acetamide augmented acylase activity. While coupled with beads entrapment, GCL consortia beads, XAL consortia beads, and urea consortia beads effectively disrupted quorum sensing (QS) and controlled membrane fouling in MBR. This work found out several optional bio-stimuli valid for tuning QQ in activated sludge system, and provided easily available and economical alternatives for QQ biostimulation, meanwhile the proposed QQ-MBR approach through QQ biostimulation and consortia entrapment also proved effective and practical.

Automated summary

This study explored various potential bio-stimuli for quorum quenching enhancement in activated sludge, identifying compounds like gamma-caprolactone, D-xylonic acid-1,4-lactone, and urea as effective in promoting inactivating activity. Through entrapment, these bio-stimuli effectively disrupted quorum sensing in membrane bioreactors.