Comparative Evaluation of Antioxidant and Anti-Inflammatory Activity of Active Compounds Identified in Ardisia elliptica Extracts from Different Plant Parts

Ardisia elliptica Thunb. (AE) has been used as food and in traditional medicine to prevent and treat fever, diarrhea, chest pain, liver poisoning, and parturition complications in Southeast Asian countries. This study focused on phytochemical constituents of AE extracts and their antioxidant and anti-inflammatory activity in vitro by evaluating nitric oxide production, and DPPH and FRAP radical scavenging activity. The bioactive compounds from different plant parts, including old leaves, young leaves, flowers, roots, and fruits, were identified. The results showed the highest phenolic and flavonoid content in the root extract among all extracts, which resulted in the most potent free radical scavenging activity revealed by the DPPH and FRAP assay. The roots and flowers showed the highest bergenin (3.36 +/- 0.22 mg/g dry weight) and quercetin (2.99 +/- 0.10 mg/g dry weight) content, respectively. In contrast, embelin was found only in the fruits. Interestingly, AE extracts significantly suppressed the mRNA expression of inducible nitric oxide synthase, leading to inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 macrophages. Conclusively, the results suggest the natural products of AE extracts as effective antioxidant and anti-inflammatory agents that can be utilized for food and pharmaceutical applications.

Automated summary

Ardisia elliptica extracts demonstrated potent antioxidant and anti-inflammatory properties, with the root extract showing the highest phenolic and flavonoid content and the most effective free radical scavenging activity. The extracts also suppressed mRNA expression of inducible nitric oxide synthase, resulting in inhibition of nitric oxide production in stimulated macrophages. The bioactive compounds in AE extracts have potential applications in food and pharmaceutical industries.