Journal
CHEMICAL ENGINEERING JOURNAL
Volume 429, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.132472
Keywords
SARS-CoV-2 RdRp gene; CRISPR; Cas12a; ECL; Triple-helix; Regenerative Biosensor; DNA tetrahedron
Categories
Funding
- National Natural Science Foundation of China [21705061]
- Jiangsu Provincial Key Medical Discipline (Laboratory) [ZDXKA2016017]
- Innovation Capacity Develop-ment Plan of Jiangsu Province [BM2018023]
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This study successfully constructed a method for SARS-CoV-2 nucleic acid detection using an exonuclease III cleavage reaction-based isothermal amplification combined with a pH-induced regenerative ECL biosensor mediated by CRISPR/Cas12a, providing ultra-sensitive and specific detection capabilities essential for large-scale screening in resource-limited areas.
In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system has good stability and reproducibility, and we expect that this method may provide a potential platform for the diagnosis of COVID-19.
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