4.7 Article

Color-convertible fluorescent nanoprobe for Parkinson's disease diagnosis

Journal

CHEMICAL ENGINEERING JOURNAL
Volume 429, Issue -, Pages -

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.132368

Keywords

Color-convertible; Nanoprobe; Fluorescent imaging; Dual-biomarker responsiveness; Parkinson's disease

Funding

  1. Shanghai Municipal Government [18JC1410800]
  2. National Key Research and Development Plan of China [2016YFA0201500]
  3. National Natural Science Foundation of China [51690151, 21875134]
  4. Yangtze River Scholar Program [WF221411002]
  5. Southern Hospital of Sixth People's Hospital affiliated to Shanghai Jiao Tong University [2017B006]
  6. 2nd Innovation Fund of Precision Medicine Union Research Center of Shanghai Jiao Tong University

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A color-convertible fluorescent nanoprobe was designed for the diagnosis of Parkinson's disease based on the high level of iron and reactive oxygen species in PD. The nanoprobe can detect dual biomarkers with high sensitivity and specificity, providing a valuable tool for PD detection in vitro and in vivo.
For the fluorescent diagnosis of Parkinson's disease (PD), molecular probes typically target a single biomarker, which is insufficient because of the limited sensitivity and specificity. Herein, by utilization of the biochemical characteristics with high level of iron and reactive oxygen species (ROS) in PD, an efficient color-convertible fluorescent nanoprobe (TAT-Polyp-QL) was designed and constructed. TAT-Polyp-QL nanoprobe contained a functional iron ligand (L1), a color-convertible fluorescent probe (Q1), and HIV-1 trans-activating transcriptor (TAT) modified hyperbranched polyphosphate (TAT-Polyp), in which TAT was employed as a tool to increase the transport efficiency of the nanoprobe across the blood-brain barrier. Upon meeting high level of iron and ROS in PD, the fluorescent probe Q1 in the TAT-Polyp-QL was oxidized into its sulfoxide derivative, resulting in the blue-shift of fluorescent emission peak from 600 nm to 550 nm and then the fluorescent color conversion from red to green. This unique reaction-based color-convertible fluorescent nanoprobe can be employed for dualbiomarker detection, identifying the emergence of PD in vitro and in vivo with high sensitivity and specificity.

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