Parahydrogen hyperpolarization of minimally altered urine samples for sensitivity enhanced NMR metabolomics

Parahydrogen hyperpolarization has been shown to enhance NMR sensitivity in urine analysis by several orders of magnitude if urine samples are prepared by solid phase extraction (SPE). We present a different approach, developed for minimal sample alteration before analysis. Removing SPE from the workflow allows to retain a wider range of metabolites and paves the way towards more universal hyperpolarized NMR metabolomics of low abundance metabolites.

Automated summary

Parahydrogen hyperpolarization significantly enhances NMR sensitivity in urine analysis when samples are prepared using solid phase extraction (SPE). A different approach that minimizes sample alteration before analysis has been proposed, allowing for a wider range of metabolites to be retained and facilitating more universal hyperpolarized NMR metabolomics of low abundance metabolites.