4.3 Review

Immunochromatography-Application Example and POCT Type Genetic Testing

Journal

CHEMICAL & PHARMACEUTICAL BULLETIN
Volume 69, Issue 10, Pages 984-988

Publisher

PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/cpb.c21-00164

Keywords

immunochromatography; point-of-care testing; PCR; infection; rapid test reagent; Coronavirus disease 2019

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Membrane-based rapid test reagents, including immunochromatography, are widely used in clinical practice. Recently, high-sensitive reagents based on immunochromatography, such as silver amplification method and time resolved fluorescence method for influenza testing, have been developed. In addition, genetic testing, which automates all steps from extraction to detection, is becoming increasingly popular, with examples like the Smart Gene Myco system for mycoplasma and COVID-19 testing.
Membrane-based rapid test reagents including immunochromatography are widely used in clinical practice. Recently, high-sensitive reagents based on the immunochromatography method, such as silver amplification method and time resolved fluorescence method for influenza testing, has been developed and early confirmation of infection can be achieved. Furthermore, genetic testing, automated all the steps from extraction till detection, is getting popular. Genetic testing of mycoplasma by Smart Gene Myco system and Coronavirus disease 2019 (COVID-19) test is a good example of membrane-based rapid test reagents. This system uses silica particle-containing membrane filter and enable to shorten the assay time by automates pre-treatment process for removing contamination substances in the sample which affect polymerase-chain-reaction amplification. We hope utilized genetic testing application will help quick confirmation of COVID-19 positive patient and prevent the collapse of medical system under COVID-19 development.

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