Journal
CHEMBIOCHEM
Volume 23, Issue 2, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202100533
Keywords
CAG; CTG repeats; DNA recognition; pyrrole-imidazole polyamides; triplet repeats
Funding
- AMED [JP21am0101101]
- JSPS KAKENHI [JP16H06356, 21H04705, 20H05936]
- NIH [RO1CA236350, 21J22276]
- Grants-in-Aid for Scientific Research [21H04705, 20H05936] Funding Source: KAKEN
Ask authors/readers for more resources
A cyclic pyrrole-imidazole polyamide (cPIP) was synthesized to bind to the abnormal expanded CAG/CTG repeat DNA sequences, showing high binding affinity and specificity for its target DNA sequence.
Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole-imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double-stranded DNA melting temperature (T-m) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next-generation sequencing showed that the cPIP had high specificity for its target DNA sequence.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
