4.4 Article

Tailoring Enzyme Stringency Masks the Multispecificity of a Lyngbyatoxin (Indolactam Alkaloid) Nonribosomal Peptide Synthetase

Related references

Note: Only part of the references are listed.
Article Microbiology

Enhanced Ohmyungsamycin A Production via Adenylation Domain Engineering and Optimization of Culture Conditions

Eunji Kim et al.

Summary: OMS A displays greater potency against Mycobacterium tuberculosis and human cancer cells compared to OMS B, with substrate promiscuous adenylation domain in the second module of OMS synthetase playing a key role in the synthesis process.

FRONTIERS IN MICROBIOLOGY (2021)

Article Multidisciplinary Sciences

Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity

Thierry Izore et al.

Summary: Non-ribosomal peptide synthetases (NRPSs) are essential for assembling complex natural products, with condensation domains playing a central role in chain assembly. Structural snapshots of a condensation domain bound to an aminoacyl-PCP substrate reveal a mechanism for controlling substrate access and tuning selectivity, without requiring a distinct pocket for side chain selection during peptide assembly.

NATURE COMMUNICATIONS (2021)

Article Chemistry, Medicinal

Mechanistic Insights into the P450 TIeB-Catalyzed Unusual Intramolecular C-N Bond Formation Involved in the Biosynthesis of Indolactam V

Jingjing Wei et al.

Summary: This study reports a computational investigation into the unique cytochrome P450 T1eB enzyme-catalyzed direct and selective C-H bond amination reaction, revealing the mechanism and key steps of this reaction.

JOURNAL OF CHEMICAL INFORMATION AND MODELING (2021)

Review Biochemistry & Molecular Biology

Bacterial terpenome

Jeffrey D. Rudolf et al.

Summary: Terpenoids are the largest and most structurally diverse family of natural products, with over 80,000 known compounds found in all domains of life. While most characterized terpenoids are produced by plants and fungi, those of bacterial origin are rare despite the biosynthetic potential present in bacteria. Despite their relatively small size, bacterial terpenoids present a fascinating dichotomy for future research due to similarities with non-bacterial terpenoids and the abundance of novel structures, biosynthetic pathways, and bioactivities.

NATURAL PRODUCT REPORTS (2021)

Article Multidisciplinary Sciences

Efficient rational modification of non-ribosomal peptides by adenylation domain substitution

Mark J. Calcott et al.

NATURE COMMUNICATIONS (2020)

Article Biochemistry & Molecular Biology

Molecular basis for the P450-catalyzed C-N bond formation in indolactam biosynthesis

Fei He et al.

NATURE CHEMICAL BIOLOGY (2019)

Review Biochemistry & Molecular Biology

Adenylation Domains in Nonribosomal Peptide Engineering

Aleksa Stanisic et al.

CHEMBIOCHEM (2019)

Article Chemistry, Multidisciplinary

A proof-reading mechanism for non-proteinogenic amino acid incorporation into glycopeptide antibiotics

Milda Kaniusaite et al.

CHEMICAL SCIENCE (2019)

Article Multidisciplinary Sciences

Context-dependent activity of A domains in the tyrocidine synthetase

Anna Degen et al.

SCIENTIFIC REPORTS (2019)

Review Biochemistry & Molecular Biology

The many faces and important roles of protein-protein interactions during non-ribosomal peptide synthesis

Thierry Izore et al.

NATURAL PRODUCT REPORTS (2018)

Article Biochemistry & Molecular Biology

Prerequisites of Isopeptide Bond Formation in Microcystin Biosynthesis

Sabine Meyer et al.

CHEMBIOCHEM (2017)

Article Biochemistry & Molecular Biology

Structures of a Nonribosomal Peptide Synthetase Module Bound to MbtH-like Proteins Support a Highly Dynamic Domain Architecture

Bradley R. Miller et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2016)

Article Multidisciplinary Sciences

Structures of two distinct conformations of holo-non-ribosomal peptide synthetases

Eric J. Drake et al.

NATURE (2016)

Article Multidisciplinary Sciences

Synthetic cycle of the initiation module of a formylating nonribosomal peptide synthetase

Janice M. Reimer et al.

NATURE (2016)

Article Chemistry, Multidisciplinary

Structural Elucidation of the Bispecificity of A Domains as a Basis for Activating Non-natural Amino Acids

Heidi Kaljunen et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015)

Article Biotechnology & Applied Microbiology

Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo

Xiaoying Bian et al.

BIOTECHNOLOGY AND BIOENGINEERING (2015)

Article Biochemistry & Molecular Biology

Improved seamless mutagenesis by recombineering using ccdB for counterselection

Hailong Wang et al.

NUCLEIC ACIDS RESEARCH (2014)

Article Biochemistry & Molecular Biology

Engineering polyketide synthases and nonribosomal peptide synthetases

Gavin J. Williams

CURRENT OPINION IN STRUCTURAL BIOLOGY (2013)

Review Biotechnology & Applied Microbiology

Specialized microbial metabolites: functions and origins

Julian Davies

JOURNAL OF ANTIBIOTICS (2013)

Article Chemistry, Multidisciplinary

Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity

Jenny Thirlway et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Review Biochemistry & Molecular Biology

Artificial Analogs of Naturally Occurring Tumor Promoters as Biochemical Tools and Therapeutic Leads

Yu Nakagawa

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2012)

Review Biotechnology & Applied Microbiology

Function of MbtH homologs in nonribosomal peptide biosynthesis and applications in secondary metabolite discovery

Richard H. Baltz

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2011)

Article Biochemistry & Molecular Biology

NRPSpredictor2-a web server for predicting NRPS adenylation domain specificity

Marc Roettig et al.

NUCLEIC ACIDS RESEARCH (2011)

Article Biochemistry & Molecular Biology

MbtH-Like Proteins as Integral Components of Bacterial Nonribosomal Peptide Synthetases

Elizabeth A. Felnagle et al.

BIOCHEMISTRY (2010)

Article Plant Sciences

Enzymatic Production of (-)-Indolactam V by LtxB, a Cytochrome P450 Monooxygenase

Minh U. Huynh et al.

JOURNAL OF NATURAL PRODUCTS (2010)

Article Chemistry, Multidisciplinary

Lyngbyatoxin biosynthesis: Sequence of biosynthetic gene cluster and identification of a novel aromatic prenyltransferase

DJ Edwards et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2004)