4.4 Article

Tailoring Enzyme Stringency Masks the Multispecificity of a Lyngbyatoxin (Indolactam Alkaloid) Nonribosomal Peptide Synthetase

Journal

CHEMBIOCHEM
Volume 23, Issue 3, Pages -

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202100574

Keywords

A domain; indolactams; MbtH; natural products; teleocidin

Funding

  1. Australian Research Council [LP140100642]
  2. Australian Government RTP Scholarship
  3. Adrian Lee Travel Scholarship
  4. Australian Research Council [LP140100642] Funding Source: Australian Research Council

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The study found that the marine cyanobacterial nonribosomal peptide synthetase (NRPS) pathway is amenable for the biosynthesis of indolactam variants, with relaxed specificity observed in the native lyngbyatoxin pathway NRPS. Site-directed mutagenesis of the substrate-binding pocket resulted in altered substrate preference, showing relative congruence between in vitro substrate activation and in vivo product formation. Further research with alternative tailoring domains may reveal the true in vivo effects of the mutations introduced.
Indolactam alkaloids are activators of protein kinase C (PKC) and are of pharmacological interest for the treatment of pathologies involving PKC dysregulation. The marine cyanobacterial nonribosomal peptide synthetase (NRPS) pathway for lyngbyatoxin biosynthesis, which we previously expressed in E. coli, was studied for its amenability towards the biosynthesis of indolactam variants. Modification of culture conditions for our E. coli heterologous expression host and analysis of pathway products suggested the native lyngbyatoxin pathway NRPS does possess a degree of relaxed specificity. Site-directed mutagenesis of two positions within the adenylation domain (A-domain) substrate-binding pocket was performed, resulting in an alteration of substrate preference between valine, isoleucine, and leucine. We observed relative congruence of in vitro substrate activation by the LtxA NRPS to in vivo product formation. While there was a preference for isoleucine over leucine, the substitution of alternative tailoring domains may unveil the true in vivo effects of the mutations introduced herein.

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