Journal
CELL STEM CELL
Volume 29, Issue 1, Pages 131-+Publisher
CELL PRESS
DOI: 10.1016/j.stem.2021.10.002
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Funding
- Max Planck Society
- ERC-Stg-2017 (VitASTEM) [759206]
- Behrens-Weise-Foundation
- German Research Foundation (DFG) under the German Excellence Strategy [CIBSS-EXC-2189, 390939984]
- German Research Foundation (DFG) [SFB1425, 422681845, SFB992, 192904750]
- European Union [813091]
- Marie Curie Actions (MSCA) [813091] Funding Source: Marie Curie Actions (MSCA)
- European Research Council (ERC) [759206] Funding Source: European Research Council (ERC)
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This study investigates the complex regulatory networks that preserve stemness in hematopoietic stem cells and uncovers a non-classical retinoic acid signaling axis that controls their function. The findings highlight the importance of a single metabolite in controlling stem cell fate through epigenetic and transcriptional attributes.
Hematopoietic stem cells (HSCs) rely on complex regulatory networks to preserve sternness. Due to the scarcity of HSCs, technical challenges have limited our insights into the interplay between metabolites, transcription, and the epigenome. In this study, we generated low-input metabolomics, transcriptomics, chromatin accessibility, and chromatin immunoprecipitation data, revealing distinct metabolic hubs that are enriched in HSCs and their downstream multipotent progenitors. Mechanistically, we uncover a non-classical retinoic acid (RA) signaling axis that regulates HSC function. We show that HSCs rely on Cyp26b1, an enzyme conventionally considered to limit RA effects in the cell. In contrast to the traditional view, we demonstrate that Cyp26b1 is indispensable for production of the active metabolite 4-oxo-RA. Further, RA receptor beta (Rarb) is required for complete transmission of 4-oxo-RA-mediated signaling to maintain stem cells. Our findings emphasize that a single metabolite controls stem cell fate by instructing epigenetic and transcriptional attributes.
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