Glutamate metabolism directs energetic trade-offs to shape host-pathogen susceptibility in Drosophila

Individual hosts within populations often show inter-individual variation in their susceptibility to bacterial pathogen-related diseases. Utilizing Drosophila, we highlight that phenotypic variation in host-pathogen susceptibility within populations is driven by energetic trade-offs, facilitated by infection-mediated changes in glutamate metabolism. Furthermore, host-pathogen susceptibility is conditioned by life history, which adjusts immunometabolic sensing in muscles to direct vitamin-dependent reallocation of host energy substrates from the adipose tissue (i.e., a muscle-adipose tissue axis). Life history conditions inter-individual variation in the activation strength of intra-muscular NF-KB signaling. Limited intra-muscular NF-KB signaling activity allows for enhanced infection-mediated mitochondrial biogenesis and function, which stimulates glutamate dehydrogenase-dependent synthesis of glutamate. Muscle-derived glutamate acts as a systemic metabolite to promote lipid mobilization through modulating vitamin B enzymatic cofactor transport and function in the adipose tissue. This energy substrate reallocation improves pathogen clearance and boosts host survival. Finally, life history events that adjust energetic trade-offs can shape inter-individual variation in host-pathogen susceptibility after infection.

Automated summary

Individual hosts within populations exhibit variation in susceptibility to bacterial pathogens, driven by energetic trade-offs and influenced by life history. Immune-metabolic sensing and vitamin-dependent energy substrate reallocation play crucial roles in modulating host-pathogen interactions, ultimately affecting pathogen clearance and host survival. Life history events can shape inter-individual variation in host-pathogen susceptibility after infection.