4.8 Article

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context

Journal

CELL METABOLISM
Volume 33, Issue 12, Pages 2464-+

Publisher

CELL PRESS
DOI: 10.1016/j.cmet.2021.11.001

Keywords

-

Funding

  1. European Research Council (ERC) [648235, 769065]
  2. European Union Marie Curie Initial Training Networks (ITN) program PerICo [812968]
  3. Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [403222702/SFB 1381, 259130777/SFB 1177, 278002225/RTG 2202, FOR 2743, PF 202/9-1, 394024777, WI 4506/1-1, 406757425, 409673687]
  4. Germany's Excellence Strategy CIBSS -EXC-2189 [390939984]
  5. European Union Marie Curie Initial Training Networks (ITN) program DRIVE [765912]
  6. European Research Council (ERC) [769065] Funding Source: European Research Council (ERC)
  7. Marie Curie Actions (MSCA) [812968] Funding Source: Marie Curie Actions (MSCA)

Ask authors/readers for more resources

Researchers have classified and identified human mitochondrial proteins, establishing a high-confidence mitochondrial proteome containing over 1,100 proteins, which account for 7% of cellular proteins and are linked to various diseases.
Mitochondria are key organelles for cellular energetics, metabolism, signaling, and quality control and have been linked to various diseases. Different views exist on the composition of the human mitochondrial proteome. We classified >8,000 proteins in mitochondrial preparations of human cells and defined a mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP). We identified interactors of translocases, respiratory chain, and ATP synthase assembly factors. The abundance of MitoCoP proteins covers six orders of magnitude and amounts to 7% of the cellular proteome with the chaperones HSP60-HSP10 being the most abundant mitochondrial proteins. MitoCoP dynamics spans three orders of magnitudes, with half-lives from hours to months, and suggests a rapid regulation of biosynthesis and assembly processes. 460 MitoCoP genes are linked to human diseases with a strong prevalence for the central nervous system and metabolism. MitoCoP will provide a high-confidence resource for placing dynamics, functions, and dysfunctions of mitochondria into the cellular context.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.8
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available