Journal
CELL COMMUNICATION AND SIGNALING
Volume 19, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12964-021-00788-x
Keywords
Desmoglein-2; E-cadherin; Helicobacter pylori; HtrA; Protease
Categories
Funding
- Austrian Science Fund (FWF) [I_4360, P_31507]
- Slovenian Research Agency (ARRS) [P1-0149, N1-0126]
Ask authors/readers for more resources
Through proteomic analysis, novel extracellular substrates of HpHtrA, including hDsg2, were identified, along with the well characterized target hCdh1. The study demonstrated efficient hDsg2 cleavage by HpHtrA, emphasizing its importance in opening intercellular junctions.
Background: High temperature requirement A (HtrA) is an active serine protease secreted by the group-I carcinogen Helicobacter pylori (H. pylori). The human cell adhesion protein and tumor suppressor E-cadherin (hCdh1) expressed on the surface of gastric epithelial cells was identified as the first HtrA substrate. HtrA-mediated hCdh1 cleavage and subsequent disruption of intercellular adhesions are considered as important steps in H. pylori pathogenesis. In this study, we performed a proteomic profiling of H. pylori HtrA (HpHtrA) to decipher the complex mechanism of H. pylori interference with the epithelial barrier integrity. Results: Using a proteomic approach we identified human desmoglein-2 (hDsg2), neuropilin-1, ephrin-B2, and semaphorin-4D as novel extracellular HpHtrA substrates and confirmed the well characterized target hCdh1. HpHtrA-mediated hDsg2 cleavage was further analyzed by in vitro cleavage assays using recombinant proteins. In infection experiments, we demonstrated hDsg2 shedding from H. pylori-colonized MKN28 and NCI-N87 cells independently of pathogen-induced matrix-metalloproteases or ADAM10 and ADAM17. Conclusions: Characterizing the substrate specificity of HpHtrA revealed efficient hDsg2 cleavage underlining the importance of HpHtrA in opening intercellular junctions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available