Iminoboronate-chitooligosaccharides hydrogels with strong antimicrobial activity for biomedical applications

The paper reports hydrogels prepared from chitooligosaccharides with different polymerization degrees (14 to 51), by crosslinking with 2-formylphenylboronicacid in three molar ratios of their functionalities. The structural, morphological and supramolecular characterization confirmed a hydrogelation mechanism based on self assembling of newly formed imine units and porous morphology. Rheological measurements confirmed the formation of thixotropic hydrogels, and swelling tests indicated mass equilibrium swelling values up to 25 in water and 9 in phosphate buffer saline. The monitoring of enzymatic degradability demonstrated the enhancing of biodegradation rate as long as the polymerization degrees of the oligomers decreased, the mass loss increasing from 16% to 43%. In vivo and ex-vivo biocompatibility investigation on experimental mice showed no cytotoxic effect, and in vitro antimicrobial tests revealed remarkable antimicrobial properties on nine strains, with a maximum inhibition diameter of 49 mm on Aspergilius brasiliensis and very good results on Cladosporium cladosporioides, Penicillium crysogenum and different Candida species.

Automated summary

The paper discusses the preparation and characterization of hydrogels from chitooligosaccharides crosslinked with 2-formylphenylboronicacid, showing promising properties in terms of biodegradability and antimicrobial activity. The results indicate that hydrogels with lower polymerization degrees exhibit higher biodegradation rates and remarkable antimicrobial properties against various strains. Further in vivo and in vitro biocompatibility tests on experimental mice demonstrate no cytotoxic effects, highlighting the potential applications of these hydrogels in the biomedical field.