Journal
CARBOHYDRATE POLYMERS
Volume 271, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118417
Keywords
Chitosan; Host-guest interaction; Nanoparticles; CpG oligodeoxynucleotides
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Alberta Livestock and Meat Agency (ALMA)
- Alberta Crop Industry Development Fund Ltd. (ACIDF)
- Alberta Innovates
- Natural Sciences and Engineering Research Council of Canada (NSERC)-Canada Research Chairs Program
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A novel chitosan nanoparticle (CS-NPs) has been designed for encapsulation and delivery of CpG ODNs, showing high encapsulation efficiency and stability. The CS-NPs can enhance the immunostimulatory activity of CpG ODNs and efficiently deliver them into living cells, making it an efficient system for treating various diseases.
CpG oligodeoxynucleotides (CpG ODNs) which can induce innate immune responses and promote adaptive immune responses, are powerful tools in defeating diseases. Here, a novel chitosan nanoparticle (CS-NPs) based on host-guest interaction has been designed for encapsulation and delivery of CpG ODNs for the first time. The CS-NPs exhibited high encapsulation efficiency (98.3%) of CpG ODNs and remained stable in storage under room temperature for at least 7 days. CS-NPs can also prevent CpG ODN diffusion at pH 7. The results of confocal laser scanning microscope images and flow cytometry show that CS-NPs can also be efficiently delivered into living cells. Furthermore, CpG@CS-NPs can increase the immunostimulatory activity of CpG ODNs. Raw 264.7 cells treated with CpG@CS-NPs demonstrated upregulation of both TNF-alpha and IL-6 cytokines by 13% and 40%, respectively. The newly developed CpG@CS-NPs were thus identified as an efficient system to deliver CpG-ODNs to treat various diseases.
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