Journal
CARBOHYDRATE POLYMERS
Volume 277, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118858
Keywords
Coated gold nanoparticles; Chitosan-collagen modified; 5-Fluorouracil; Layer-by-layer assembly technique; Biotin; Reduction reaction
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Funding
- Research Assistantship Fund, Faculty of Science, Chulalongkorn University, Thailand
- National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand, through its program of Research Network NANOTEC (RNN)
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In this study, novel nanohybrids of gold nanoparticles coated with collagen and modified with BiQCS were developed for improved drug delivery. The layer-by-layer technique increased the drug loading capacity of the nanoparticles and enhanced their cellular uptake and specific targeting. The nanohybrids also exhibited strong anti-inflammatory activity and greatly improved the activity of the drug. These findings suggest that the Bi-QCSAuNPs@collagen could be a promising drug delivery carrier in the future.
We report herein the development of the novel nanohybrids of gold nanoparticles reduced/stabilized/coated with collagen (AuNPs@collagen) in the first layer and subsequently modified with biotin-quat188-chitosan (BiQCS) in the outer layer for 5-fluorouracil (5-FU) delivery to improve cellular uptake and promote specific cell targeting of the nanocarrier. The fabrication of the layer-by-layer technique on the surface of gold nanoparticles (AuNPs) can overcome the limitation of poor drug loading capacity of the classic AuNPs from 64.67% to 87.46%. The AuNPs@collagen coated by the Bi-QCS exhibits strong electrostatic interactions between drug anion (5-FU) and amine groups of the modified chitosan as well as hydrogen bonding. Furthermore, the Bi-QCSAuNPs@collagen demonstrated a significantly higher anti-inflammatory activity in RAW264.7 macrophage cell line. The Bi-QCS-AuNPs@collagen enhanced the activity of 5-FU approximately 3.3-fold (HeLa) and 6.2-fold (A549), compared to the free 5-Fluorouracil. According to these results, it is very promising that Bi-QCSAuNPs@collagen can be used as an effective drug delivery carrier in the future.
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