Journal
CARBOHYDRATE POLYMERS
Volume 270, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118316
Keywords
Alginate oligosaccharide; Intestinal barrier; Escherichia coli; Lipopolysaccharide; Inflammatory injury
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Funding
- National Natural Science Foundation of China [31972599]
- Development Program of Sichuan Province [20ZDYF0003]
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The study demonstrates that alginate oligosaccharide can protect the intestinal mucosa from inflammatory injury induced by Escherichia coli and achieve this effect by inhibiting the activation of NF-kappa B.
Alginate oligosaccharide (AOS) possesses various pharmaceutical benefits, making it an attractive candidate for biomedical applications. In the present study, we prepared AOS by depolymerising alginate; its degree of polymerisation mainly ranged from 2 to 8. We confirmed the enteroprotective potential of AOS against enterotoxigenic Escherichia coli (ETEC)-induced intestinal barrier injury in weaned pigs. Next, we illustrated the mechanisms underlying this effect of AOS using the porcine small intestinal epithelial cell line IPEC-J2. AOS potently reduced the binding of the bacteria-deprived endotoxin lipopolysaccharide (LPS) to the IPEC-J2 cell surface. Moreover, it suppressed the LPS-induced production of pro-inflammatory cytokines and the nuclear translocation of nuclear factor-kappa B (NF-kappa B) p65 in IPEC-J2 cells. These results indicate that AOS protects the intestinal epithelium from ETEC-induced inflammatory injury by preventing the activation of NF-kappa B, implying that AOS could be used as an anti-inflammatory agent for treating inflammation-related intestinal diseases in mammals.
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