A dandelion polysaccharide and its selenium nanoparticles: Structure features and evaluation of anti-tumor activity in zebrafish models

In this study, an inulin fructan (TMP50-2) with moderate anti-tumor activity was obtained from dandelion. To further improve the anti-tumor activity of TMP50-2, a monodisperse and stable spherical nanoparticle (Tw-TMP-SeNP, 50 nm) was fabricated. Physico-chemical analysis revealed that TMP50-2 and Tween 80 were tightly wrapped on the surface of SeNPs by forming C-O center dot center dot center dot Se bonds or through hydrogen bonding interaction (O-H center dot center dot center dot Se). In vitro anti-tumor assay showed that Tw-TMP-SeNP treatment could significantly inhibit the proliferation of cancer cells (HepG2, A549, and HeLa) in a dose-dependent manner, while HepG2 cells were more susceptible to Tw-TMP-SeNP with an IC50 value of 46.8 mu g/mL. The apoptosis induction of HepG2 cells by Tw-TMP-SeNP was evidenced by increasing the proportion of apoptotic cells ranging from 12.5% to 27.4%. Furthermore, in vivo zebrafish model confirmed the anti-tumor activity of Tw-TMP-SeNP by inhibiting the proliferation and migration of tumor cells as well as the angiogenesis of zebrafish embryos.

Automated summary

In this study, a monodisperse spherical nanoparticle Tw-TMP-SeNP was fabricated to enhance the anti-tumor activity of inulin fructan TMP50-2 obtained from dandelion. Both in vitro and in vivo experiments confirmed the significant inhibitory effect of Tw-TMP-SeNP on the proliferation, migration, and angiogenesis of cancer cells, highlighting its potential as a treatment for various cancers.