Journal
CARBOHYDRATE POLYMERS
Volume 277, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2021.118822
Keywords
Carboxylated chitosan; EGF receptors (EGFR; HER2); Aptamer; Triple negative breast cancer; HER2 positive breast cancer; Mesoporous silica nanoparticle; Nanoparticulate drug delivery
Categories
Funding
- Department of Biotechnology, Government of India (GoI) [BT/PR21221/NNT/28/1069/2016]
- MHRD, GoI
- Mehta Family Centre for Engineering in Medicine
- IIT Kanpur
Ask authors/readers for more resources
In this study, a chitosan-coated nanoparticle was developed for targeting breast cancer cells, showing higher cellular uptake and cytotoxicity compared to non-targeted nanoparticles. The chitosan coating provided pH-responsiveness and endo/lysosomal escape ability to the nanoparticles, enhancing the efficacy of anticancer drug delivery.
Nanoparticle-based targeting of overexpressed cell-surface receptors is a promising strategy that provides precise delivery of drugs to cancer cells. In the present study, we developed highly reproducible and monodispersed, chitosan-coated (pH-responsive), doxorubicin-loaded, aptamer-mesoporous silica nanoparticle (MSN) bioconjugates for actively targeting breast cancer cells harboring overexpression of EGF receptors (EGFR/HER2). The developed targeted MSNs demonstrated higher uptake and cytotoxicity of triple negative and HER2 positive breast cancer cells when compared to non-targeted MSNs. The chitosan coating imparted pH-responsiveness and endo/lysosomal escape ability to MSNs, which augmented cytosolic delivery of an anticancer drug. Partial carboxylation of chitosan coated on MSNs allowed for a greater release of drug in a shorter duration of time while retaining pH-responsiveness and endo/lysosomal escape ability. Overall, the coating of carboxylated-chitosan over MSNs enabled tunable drug release kinetics, conjugation of aptamers (targeting agents), and endo/lysosomal escape which together significantly enhanced the efficacy of the developed drug delivery system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available