A strategy for controlling Cercospora leaf spot, caused by Cercospora beticola, by combining induced host resistance and chemical pathogen control and its implications for sugar beet yield

Cercospora beticola populations resistant to methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), and demethylation inhibitor (DMI) fungicides have been reported in Egypt. This pathogen causes a difficult to control variant of Cercospora leaf spot (CLS) on sugar beet. We conducted field experiments over two years to investigate treatment efficacies on MBC- and QoI-resistant, but partially DMI-sensitive, C. beticola from a research farm in Sakha, Egypt. DMI fungicides showed moderate efficacy with half the maximal effective concentration (EC50). Efficacy was 67.2-69.1% and 63.4-63.6% for epoxiconazole (EPO) and propiconazole (PRO), respectively. When each fungicide was mixed with salicylic acid (SA) at EC50 (EPO + SA) and EC50 (PRO +SA), efficacy was 77.5-79.1% and 77.0-78.2%, respectively. The QoI fungicide, azoxystrobin, showed lower efficacy (46.4-49.5%) when applied alone, but enhanced efficacy when combined with SA at EC50/EC50 (77.0-78.2%). Carbendazim alone was only 47.5-45.1% effective, but when mixed with SA, had a 67.1% co-toxicity factor in vitro, indicating antagonism between components. An Artemisia cina aqueous extract (WEA) combined with SA (WEA/SA) showed 76.0-78.2% efficacy, compared with 69.0-71.0% and 49.0-52.7% for WEA and SA, respectively. Single treatments were classified into five sensitivity classes from sensitive (<1) to resistant (>100) based on their EC50 values. Significant increases in insensitivity were observed from 2015/16 to 2017/18. All treatments increased total yield, sucrose percentage, and recoverable white sugar compared with the untreated control. A combination of control strategies could allow for effective disease and fungicide resistance management.

Automated summary

This study found that the pathogen causing Cercospora leaf spot on sugar beet in Egypt has developed resistance to certain fungicides, but remains sensitive to others. The efficacy of fungicides was improved when used in combination with salicylic acid, but antagonistic effects were observed when certain fungicides were mixed together. Additionally, the study showed that combining multiple control strategies can increase yield and sucrose percentage.