Journal
BREEDING SCIENCE
Volume 72, Issue 2, Pages 150-168Publisher
JAPANESE SOC BREEDING
DOI: 10.1270/jsbbs.21081
Keywords
iTRAQ-labeling; quantitative proteomics; cold stress; japonica rice
Categories
Funding
- National Natural Science Foundation of China [31960059, 31960401, U20A2032]
- Guangxi Natural Science Foundation [2017GXNSFAA198266, 2018GXNSFAA050128]
- Guangxi Science and Technology Base and Special Talents [GuiKe AD18281069, GuiKe AD18050002, GuiKe AD17129064]
- Guangxi Academy of Agricultural Science [31960059, 2021JM23, GuiNongKe2020YM124]
- Guangxi Talent High Land of High Quality Breeding Research [Talent High Land QN-27]
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By using the iTRAQ proteomics approach, we investigated the response of rice to low temperature stress. In this study, we detected changes in multiple proteins and found that the regulated proteins are mainly involved in photosynthesis, metabolic pathway, biosynthesis of secondary metabolites, and carbon metabolism. Additionally, we observed that mRNA levels do not always correspond to protein levels. This study provides new insights into cold stress responses in rice with respect to proteomics and identifies candidate genes for cold-tolerance rice breeding.
Low temperature is one of the important environmental factors that affect rice growth and yield. To better understand the japonica rice responses to cold stress, isobaric tags for a relative and absolute quantification (iTRAQ) labeling-based quantitative proteomics approach was used to detected changes in protein levels. Two-week-old seedlings of the cold tolerant rice variety Kongyu131 were treated at 8 degrees C for 24, 48 and 72 h, then the total proteins were extracted from tissues and used for quantitative proteomics analysis. A total of 5082 proteins were detected for quantitative analysis, of which 289 proteins were significantly regulated, consisting of 169 uniquely up-regulated proteins and 125 uniquely down-regulated proteins in cold stress groups relative to the control group. Functional analysis revealed that most of the regulated proteins are involved in photosynthesis, metabolic pathway, biosynthesis of secondary metabolites and carbon metabolism. Western blot analysis showed that protein regulation was consistent with the iTRAQ data. The corresponding genes of 25 regulated proteins were used for quantitative real time PCR analysis, and the results showed that the mRNA level was not always parallel to the corresponding protein level. The impor-tance of our study is that it provides new insights into cold stress responses in rice with respect to proteomics and provides candidate genes for cold-tolerance rice breeding.
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