4.7 Article

Transcriptomic and metabolomic changes triggered by Macrosiphum rosivorum in rose (Rosa longicuspis)

Journal

BMC GENOMICS
Volume 22, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12864-021-08198-6

Keywords

Rose; Aphid; Glutathione metabolism; Glucosinolate metabolism

Funding

  1. National Key R&D Program of China [2018YFD1000400]
  2. National Natural Science Foundation of China [31860571, 31560565]
  3. Major Science and Technology Projects Yunnan Province [2016ZA005]
  4. youth top-notch talents training project of 'ten thousand talents plan' of Yunnan Province
  5. leading talents of Yunling industrial technology
  6. Major agricultural science and technology projects in Yunnan Province [202102AE090052]
  7. Yunnan academician expert workstation [202105AF150036]

Ask authors/readers for more resources

In this study, M. rosivorum quickly colonized R. longicuspis after inoculation. Under M. rosivorum stress, R. longicuspis responded by activating MAPK cascades, plant hormone signal transduction pathway, RlMYBs and RlERFs transcription factors expression, and ROS production. The 'glutathione metabolic' and 'glucosinolate biosynthesis' pathways played significant roles in rose resistance against aphids.
Background Rose is one of the most popular flowers in the wold. Its field growth and quality are negatively affected by aphids. However, the defence mechanisms used by rose plants against aphids are unclear. Therefore, to understand the defence mechanism of rose under aphid stress, transcriptome and metabolome techniques were used to investigate the regulation mechanism in R. longicuspis infected with M. rosivorum. Result In our study, after inoculation with M. rosivorum, M. rosivorum quickly colonized R. longicuspis. A total of 34,202 genes and 758 metabolites were detected in all samples. Under M. rosivorum stress, R. longicuspis responded by MAPK cascades, plant hormone signal transduction pathway activation, RlMYBs and RlERFs transcription factors expression and ROS production. Interestingly, the 'brassinosteroid biosynthesis' pathway was significantly enriched in A3 d-vs.-A5 d. Further analysis showed that M. rosivorum induced the biosynthesis of secondary metabolites such as terpenoids, tannins and phenolic acids, among others. Importantly, the 'glutathione metabolic' and 'glucosinolate biosynthesis' pathways were significantly enriched, which involved in the rose against aphids. Conclusion Our study provides candidate genes and metabolites for Rosa defence against aphids. This study provides a theoretical basis for further exploring the molecular regulation mechanism of rose aphid resistance and aphid resistance breeding in the future.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available