Journal
BMC CANCER
Volume 21, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12885-021-08812-6
Keywords
miR-17-5p; HNSCC; Cell cycle; CCNG2; Recurrence
Categories
Funding
- National Natural Science Foundation of China [81972529]
- Science and Technology Commission of Shanghai Municipality [19411961300]
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In this study, it was found that miR-17-5p expression was significantly increased in HNSCC tissues and cell lines, and it is associated with recurrence in HNSCC patients. Silencing miR-17-5p blocked HNSCC cells in G2/M phase, while its overexpression promoted cell cycle progression. It was confirmed that miR-17-5p negatively regulated CCNG2 mRNA and protein expression by directly targeting its 3'UTR.
Background The human miR-17-92 polycistron is the first reported and most well-studied onco-miRNA with a cluster of seven miRNAs. miR-17-5p, a member of the miR-17-92 family, plays an important role in tumor cell proliferation, apoptosis, migration and invasion. However, few studies have shown the role of miR-17-5p in the cell cycle of head and neck squamous cell carcinoma (HNSCC). Methods RT-qPCR was used to detect miR-17-5p expression levels in 64 HNSCC tissues and 5 cell lines. The relationship between the expression of miR-17-5p in the tissues and the clinical characteristics of the patients was analyzed. HNSCC cells were transfected with an miR-17-5p mimic or inhibitor to evaluate cell cycle distribution by flow cytometry. Cell cycle distribution of cells transfected with target gene was evaluated using flow cytometry. Dual-luciferase reporter assay was used to detect the regulatory effect of miR-17-5p on target gene expression. Results In the present study, we found that miR-17-5p expression in HNSCC tissues and cell lines was remarkably increased, and miR-17-5p is related to recurrence in HNSCC patients. Silencing miR-17-5p blocked HNSCC cells in G2/M phase, whereas its overexpression propelled cell cycle progression. More importantly, we verified that miR-17-5p negatively regulated CCNG2 mRNA and protein expression by directly targeting its 3'UTR. Conclusion These findings suggest that miR-17-5p might act as a tumor promoter and prognostic factor for recurrence in HNSCC patients.
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