Journal
BIOTECHNOLOGY LETTERS
Volume 44, Issue 1, Pages 33-44Publisher
SPRINGER
DOI: 10.1007/s10529-021-03208-9
Keywords
Endonucleases; Methylation; Methyltransferase; Restriction-modification system
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Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2018R1A5A1025077]
- Chung-Ang University
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The prokaryotic restriction-modification (RM) system protects the host by cleaving foreign DNA, making it difficult to introduce engineered plasmid DNAs into newly isolated microorganisms. Methyltransferases can be utilized to methylate engineered plasmid DNAs before transformation, enhancing stability within cells. Study on methyltransferases in newly isolated bacteria is essential for genetic engineering.
Since prokaryotic restriction-modification (RM) systems protect the host by cleaving foreign DNA by restriction endonucleases, it is difficult to introduce engineered plasmid DNAs into newly isolated microorganisms whose RM system is not discovered. The prokaryotes also possess methyltransferases to protect their own DNA from the endonucleases. As those methyltransferases can be utilized to methylate engineered plasmid DNAs before transformation and to enhance the stability within the cells, the study on methyltransferases in newly isolated bacteria is essential for genetic engineering. Here, we introduce the mechanism of the RM system, specifically the methyltransferases and their biotechnological applications. These biotechnological strategies could facilitate plasmid DNA-based genetic engineering in bacteria strains that strongly defend against foreign DNA.
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