Journal
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
Volume 27, Issue 1, Pages 135-144Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-020-0393-y
Keywords
MIL-125-NH2; amino nationalization; enzyme immobilization; covalent binding
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Funding
- Science and Technology Program of Tianjin, China [20ZYJDJC00080]
- Key Projects of Tianjin Natural Science Foundation, China [19JCZDJC38100]
- Key R&D projects in Zhongning County, China [2021YBYF0808]
- project of Guangxi Key Research and Development program, China [GuikeAB21238005]
- project of Key Research and Development of Shandong province, China [2021CXGC010509]
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In this study, catalase was immobilized on amino functionalized MIL-125-NH2 by adsorption and covalent binding methods, displaying higher activity recovery and stability compared to free catalase. The immobilized catalase showed good reusability after multiple uses, making amino functionalized MIL-125-NH2 an excellent carrier for enzyme immobilization.
As a series of metal organic framework (MOFs), materials of institute lavoisier frameworks (MILs) are expected to be excellent supports for enzyme immobilization due to their good water-stability and acid tolerance. However, enzyme loading on MIL-125-NH2 is very low due to small pore size and few amino groups on the surface of the MIL-125-NH2. In this work, catalase (CAT) was immobilized on the MIL-125-NH2 and amino functionalized MIL-125-NH2 by adsorption (CAT@MIL-125-NH2) and covalent binding (CAT@Amino MIL-125-NH2), respectively. Compared with the CAT@MIL-125-NH2 and free CAT, the CAT@Amino MIL-125-NH2 displayed high activity recovery, good pH stability, stability against denaturants, and thermostability. Furthermore, activity recovery of CAT@Amino MIL-125-NH2 was 56% higher than CAT@MIL-125-NH2. The CAT@Amino MIL-125-NH2 still retained 50% residual activity for 14 days at room temperature, whereas free CAT lost activity after storage for 1 day at the same storage conditions. Furthermore, the CAT@Amino MIL-125-NH2 maintained 62% of its initial activity after 4 consecutive uses, showing good reusability. The results showed that the amino functionalized MIL-125-NH2 is an excellent carrier of enzyme immobilization.
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