Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 119, Issue 4, Pages 1142-1156Publisher
WILEY
DOI: 10.1002/bit.28047
Keywords
adipose-derived stem cells; biomaterial; cell-derived matrix; decellularization; extracellular matrix
Categories
Funding
- Peter and Traudl Engelhorn Foundation
- Ministry of Science, Research and the Arts, Baden-Wurttemberg, Germany
- German Science Foundation [INST37/829-1FUGG]
Ask authors/readers for more resources
This study compared and characterized human adipose tissue-derived dECM and cdECM, revealing differences in molecular composition, structural characteristics, and biological purity. These findings can aid researchers in selecting suitable ECM-based biomaterials for specific applications and interpreting experimental results.
Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively toward their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells, toward their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans compared with cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available