4.6 Article

On-chip magnetophoretic capture in a model of malaria-infected red blood cells

Journal

BIOTECHNOLOGY AND BIOENGINEERING
Volume 119, Issue 4, Pages 1129-1141

Publisher

WILEY
DOI: 10.1002/bit.28030

Keywords

lab-on-a-chip; malaria; magnetorphoretic separation

Funding

  1. Politecnico di Milano
  2. H2020 European Research Council [646990 -NICHOID]

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This study systematically characterizes an easy-to-operate lab-on-chip for the magnetophoretic capture of malaria-infected red blood cells. The method achieves high capture efficiency in a short amount of time and requires simple sample preparation.
The search for new rapid diagnostic tests for malaria is a priority for developing an efficient strategy to fight this endemic disease, which affects more than 3 billion people worldwide. In this study, we characterize systematically an easy-to-operate lab-on-chip, designed for the magnetophoretic capture of malaria-infected red blood cells (RBCs). The method relies on the positive magnetic susceptibility of infected RBCs with respect to blood plasma. A matrix of nickel posts fabricated in a silicon chip placed face down is aimed at attracting infected cells, while healthy cells sediment on a glass slide under the action of gravity. Using a model of infected RBCs, that is, erythrocytes with methemoglobin, we obtained a capture efficiency of about 70% after 10 min in static conditions. By proper agitation, the capture efficiency reached 85% after just 5 min. Sample preparation requires only a 1:10 volume dilution of whole blood, previously treated with heparin, in a phosphate-buffered solution. Nonspecific attraction of untreated RBCs was not observed in the same time interval.

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