Evaluation of growth and some unexplored bioactivities of bioreactor grown adventitious root culture of ginseng (Panax ginseng CA Meyer)

The purpose of the present study was to evaluate the growth potential and some rarely reported bioactivities (antioxidant, thrombolytic, anticoagulant, and anthelmintic) of Panax ginseng C.A. Meyer adventitious roots. To demonstrate the growth, shake flask and laboratory-scale bioreactor cultures have been employed. The obtained biomass was dried and extracted with water, ethanol, and methanol. The growth ratio (12.62 +/- 1.03) observed in the bioreactor was significantly higher than in the shake flask culture. The presence of 10 different phytochemical classes, including carbohydrates, saponins, glycosides, and terpenoids were detected in qualitative estimation. Significant quantities of phenolics, flavonoids, proteins, and tannins were determined. Dose-dependent antioxidant activities were observed, and the IC50 values of methanolic and ethanolic extracts were very similar to the standard. The highest (29.26 +/- 5.31%) thrombolytic potential was shown by the methanolic extract. The ethanolic extract significantly extended the coagulation times up to 2.5 fold. The highest anthelmintic properties in terms of paralyzing (2.21 +/- 0.31 min) and killing (3.69 +/- 0.41 min) of the parasitic worms were displayed by the aqueous extract. The in vitro root growth implies the commercial feasibility of ginseng production in Bangladesh and the demonstration of potential bioactivities strengthens medicinal implications and also offering new research areas.

Automated summary

The study evaluated the growth potential and various bioactivities of Panax ginseng C.A. Meyer adventitious roots, including antioxidant, thrombolytic, anticoagulant, and anthelmintic properties. The use of laboratory-scale bioreactor resulted in higher growth ratio compared to shake flask culture, with qualitative estimation revealing multiple phytochemical classes in the extracts. Dose-dependent antioxidant activities were observed, along with significant thrombolytic and anthelmintic properties displayed by different extracts. The results imply commercial feasibility for ginseng production in Bangladesh and indicate potential medicinal implications for further research.