Journal
BIOTECHNIQUES
Volume 72, Issue 2, Pages 65-72Publisher
FUTURE SCI LTD
DOI: 10.2144/btn-2021-0104
Keywords
Allan variance; horizontal magnetic tweezers; magnetic traps; magnetic tweezers; particle tracking; power spectrum; single-molecule micromanipulation; vertical magnetic tweezers; video microscopy
Funding
- Catholic University of America
- Vitreous State Laboratory
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The use of magnetic tweezers in single-molecule manipulation has rapidly evolved in the past 30 years, providing important insights into the dynamic activity of DNA-processing enzymes and the mechanical properties of DNA. These advancements have enabled a richer mechanistic understanding of DNA-binding enzymes and their functions, making magnetic tweezers a superlative tool for investigating DNA interactions at the single-molecule level.
The use of magnetic tweezers for single-molecule micromanipulation has evolved rapidly since its introduction approximately 30 years ago. Magnetic tweezers have provided important insights into the dynamic activity of DNA-processing enzymes, as well as detailed, high-resolution information on the mechanical properties of DNA. These successes have been enabled by major advancements in the hardware and software components of these devices. These developments now allow for a much richer mechanistic understanding of the functions and mechanisms of DNA-binding enzymes. In this review, the authors briefly discuss the fundamental principles of magnetic tweezers and describe the advancements that have made it a superlative tool for investigating, at the single-molecule level, DNA and its interactions with DNA-binding proteins.
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