Journal
BIOSENSORS & BIOELECTRONICS
Volume 193, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2021.113523
Keywords
Aptamers; SELEX; Krait; Snake venom; Bungarotoxin; Mass-spectrometry; Paper device
Categories
Funding
- THSTI Core grant
- Department of Biotechnology, Govt. of India [BT/010/IYBA/2016/10]
- BIRAC-PACE grant [BT/AIR0340/PACE-13/17]
- European Union Seventh Framework Programm [PIRSES-GA-2013-612,131]
- Rufford Foundation
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The study successfully developed a panel of highly affine aptamers from Bungarus caeruleus venom using SELEX technology, which specifically recognizes the B. caeruleus venom and distinguishes it from other snake venoms. The aptamer-based paper device can detect very low concentrations of krait venom and has potential for point-of-care venom detection applications.
Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is beta-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.
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