Journal
BIORESOURCE TECHNOLOGY
Volume 338, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125565
Keywords
Sugarcane bagasse; Pediococcus pentosaceus; Bacteriocin; Hemicellulose hydrolysate
Funding
- Sao Paulo Research Foundation (FAPESP) [2013/07914-8, 2015/24777-0, 2018/04385-8, 2018/25511-1]
- National Council for Scientific and Technological Development (CNPq)
- Coordination for the Improvement of Higher Education Personnel (CAPES) [001]
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This study successfully produced a purified pediocin with a purity exceeding 95% using bacteriocin produced from hemicellulose hydrolysate of sugarcane bagasse. By optimizing culture conditions and purification steps, the research provided an effective approach for pediocin production.
Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg.L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.
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