Journal
BIORESOURCE TECHNOLOGY
Volume 344, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.126187
Keywords
Microalga; Heterotrophic Chlorella; Two-stage cultivation; Starch; protein
Funding
- National Natural Science Foundation of China, China [32172997]
- Key Projects of Department of Science and Technology of Fujian Province of China, China [2020N0008, 2019N0011]
- Innovation Training Program for College Students of Fujian Province, Chnia [S202110394035]
- Scientific Research Innovation Program Xiyuanjiang River Scholarship, China (College of Life Sciences, Fujian Normal University)
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The study established an efficient two-stage cultivation process to produce microalgal biomass rich in protein by optimizing nutrient conditions and gene expression. Chlorella sp. MBFJNU-17 showed promising potential for producing high-value biomass enriched in protein.
This work aimed to firstly establish an efficient and novel two-stage cultivation process to produce microalgal biomass rich in protein using a heterotrophic Chlorella sp. MBFJNU-17 strain. In the first-stage cultivation, to reduce the glucose and urea utilization, microalga achieved a high biomass at 40 g/L glucose and 1 g/L urea; meantime, the expression from starch biosynthesis genes of microalga was up-regulated under nitrogenstarvation conditions for starch accumulation (55.01%). In the second-stage cultivation, based on the overcompensation effect, Chlorella cells after the first-stage cultivation were further treated at 5 g/L glucose and 3 g/L urea to up-regulate starch degradation, central carbon metabolism and urea absorption genes expression to drive intracellular starch-to-protein switch for biosynthetic protein (59.75%). Moreover, microalga performed similar characteristics in a 10-L fermenter by the established process. Taken together, Chlorella sp. MBFJNU-17 was the promising candidate to produce high-value biomass enriched in protein by the established two-stage cultivation.
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