Simultaneous nitrification and aerobic denitrification by a novel isolated Ochrobactrum anthropi HND19

The study aimed to isolate a novel strain with heterotrophic nitrification and aerobic denitrification ability and evaluate the nitrogen removal characteristics. Results showed that Ochrobactrum anthropi HND19 could remove approximately 98.6% of NH4+-N (104.3 mg.L-1) and 97.6% of NO3--N (98.6 mg.L-1 ), and the removal rates achieved 4.28 and 4.01 mg-N/(L.h) by heterotrophic nitrification and aerobic denitrification. The optimal incubate conditions of strain HND19 were 120 rpm (shaking speed), 5 parts per thousand (salinity), 30 degrees C (temperature), 7.5 (C/N ratio) with sodium acetate as carbon resource. And the removal efficiency of the total nitrogen (TN) realized 73.4% under the optimal conditions. Functional genes (hao, napA, nirK, norB, and nosZ) involved in the nitrogen removal processes were successfully amplified from strain HND19. These findings indicate that the strain HND19 possesses great application feasibility in treating wastewater with high-intensity nitrogen.

Automated summary

The study isolated a novel strain, HND19, with high efficiency in nitrogen removal, achieving a removal rate of 73.4% for total nitrogen under optimal conditions. Functional genes involved in nitrogen removal processes were successfully amplified in strain HND19, indicating its potential application in treating wastewater with high-intensity nitrogen.