Journal
BIORESOURCE TECHNOLOGY
Volume 337, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125463
Keywords
Xylose; Enterobacter ludwigii; Aqueous two-phase system; Ammonium sulphate; Isopropanol; 2; 3-butanediol
Funding
- vWa Project [BB/S011951/1]
- BBSRC
- Innovate UK
- Department of Biotechnology, India
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The study focused on the recovery and purification of 2,3-Butanediol (BDO) produced from detoxified xylose-rich sugarcane bagasse hydrolysate by a mutant strain of Enterobacter ludwigii, with successful extraction of BDO in isopropanol using optimized aqueous-two phase system.
Downstream processing of chemicals obtained from fermentative route is challenging and cost-determining factor of any bioprocess. 2,3-Butanediol (BDO) is a promising chemical building block with myriad applications in the polymer, food, pharmaceuticals, and fuel sector. The current study focuses on the recovery and purification of BDO produced (68.2 g/L) from detoxified xylose-rich sugarcane bagasse hydrolysate by a mutant strain of Enterobacter ludwigii. Studies involving screening and optimization of aqueous-two phase system (ATPS) revealed that 30% w/v (NH4)2SO4 addition to clarified fermented broth facilitated BDO extraction in isopropanol (0.5 v/v), with maximum recovery and partition coefficient being 97.9 +/- 4.6% and 45.5 +/- 3.5, respectively. The optimized protocol was repeated with unfiltered broth containing 68.2 g/L BDO, cell biomass, and unspent protein, which led to the partitioning of 66.7 g/L BDO, 2.0 g/L xylose and 9.0 g/L acetic acid into organic phase with similar BDO recovery (97%) and partition coefficient (45).
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