Effects of chlorine disinfectants on the microbial community structure and the performance of anaerobic digestion of swine manure

The residual chlorine disinfectants (CDs) in swine slurry could negatively impact the anaerobic digestion (AD). The objective of this study was to investigate the effects of CDs on mesophilic and thermophilic AD. The results indicated that CDs exerted inhibition effects on methanogenesis at the initial stage of mesophilic AD, leading to the extension of lag time from 0.62 days for control to 0.85, 1.9, 3.8, and 5.5 days with the increasing CDs concentrations of 50, 100, 200, and 400 mg/L, respectively. Under thermophilic condition, the inhibition effects reduced significantly at the initial stage but a decrease of CMPu at later stage was observed. The microbial analysis revealed that CDs resulted in the enrichment of chlorine-resistant bacteria (Clostridum_sensu_stricto_1) and archaea (Methanosarcina). Addition of activated carbon (AC), zero-valent iron (ZVI) and biochar (BC) was evaluated for alleviating the inhibitions of CDs and proved to be feasible strategies to alleviate the inhibited AD.

Automated summary

Residual chlorine disinfectants can inhibit anaerobic digestion, particularly in the initial stage of mesophilic conditions. Inhibitory effects were reduced in thermophilic conditions, but a decrease in CMPu was observed later. Microbial analysis revealed enrichment of chlorine-resistant bacteria and archaea due to the presence of residual chlorine disinfectants.