Driving factors of nitrogen conversion during chicken manure aerobic composting under penicillin G residue: Quorum sensing and its signaling molecules

This study explored effects of different concentrations of penicillin G on nitrogen conversion, bacterial community composition, and quorum sensing during chicken manure aerobic composting. After composting, adding penicillin G down-regulated the abundance of 71 genera and up-regulated the abundance of 103 genera. These bacterial genera were mainly Firmicutes and Proteobacteria. 16S rRNA gene sequencing was employed for function prediction, and the results showed that the addition of penicillin G increased nitrification, reduced denitrification. The autoinducer-1 (AI-1), autoinducer-3 (AI-3) and Phr signal molecules further participated in the nitrogen cycle by regulating the population behavior among multiple bacterial genera. In addition, SEM analysis showed that the quorum sensing system negatively regulated the abundance of genus related to the nitrogen conversion during chicken manure aerobic composting. This is a new theoretical analysis of the research on the treatment of hazardous materials.

Automated summary

This study investigated the effects of different concentrations of penicillin G on nitrogen conversion, bacterial community composition, and quorum sensing during chicken manure aerobic composting. The addition of penicillin G was found to impact the abundance of specific bacterial genera, predominantly from Firmicutes and Proteobacteria phyla. Furthermore, the study demonstrated that penicillin G increased nitrification and reduced denitrification, while the quorum sensing system played a negative regulatory role in the nitrogen conversion process.