Journal
BIORESOURCE TECHNOLOGY
Volume 338, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125571
Keywords
Chlorella; Initial pH; Photosynthetic bacteria; Bio-hydrogen
Funding
- National Key R&D Program of China [2018YFE0206600]
- National Natural Science Foundation of China [52076068]
- Henan International Joint Laboratory of Biomass Energy and Nanomaterials
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The study investigated the influence of different enzyme additions on bio-hydrogen production from Chlorella by photosynthetic organisms, finding that the mixed enzyme hydrolysis resulted in better bio-hydrogen production. This provides a low-cost bio-hydrogen technology option.
In the paper, Use Chlorella as raw material, HAU-Ml Photosynthetic bacteria (PSB) as hydrogen-producing bacteria, the influence of initial pH on bio-hydrogen by photosynthetic organisms from Chlorella vulgaris with diverse enzyme addition was studied. The results showed that when using cellulase as hydrolase, the optimum initial pH was 7.0 and highest bio-hydrogen was 25.99 mL/g dry cell weight. Using neutral protease as hydrolase, the optimum initial pH was 8.0 and highest bio-hydrogen was 16.47 mL/g dry cell weight. Using mixed enzyme of cellulase and protease as hydrolase, the optimal initial pH was 7.0 and highest bio-hydrogen was 27.43 mL/g dry cell weight. The bio-hydrogen from Chlorella after mixed enzymatic hydrolysis is better than that of single enzymatic hydrolysis, we think the mixed enzymatic hydrolysis of cellulase and protease was superior to the single enzymatic hydrolysis of the two enzymes, which provides a scientific reference and low-cost bio-hydrogen technology by microalgae.
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