Journal
BIORESOURCE TECHNOLOGY
Volume 338, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2021.125564
Keywords
Lignin; Valorization; Enzymes; Ionic liquid; Biocompatibility
Funding
- Nebraska Agricultural Research Division
- Nebraska Center for Energy Science Research
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2014/18714-2, 2017/50025-0]
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This study developed a novel approach to combine aqueous lignin extraction with enzymatic lignin depolymerization in biocompatible ionic liquids, which successfully reduced lignin molecular weight.
Enzymatic depolymerization of lignin to produce low molecular weight products requires mild reaction conditions and exhibits higher selectivity compared to thermochemical lignin depolymerization. However, it remains challenging to depolymerize lignin enzymatically, partially due to the low solubility of lignin in aqueous phase. This study aimed to develop a novel approach to combine aqueous lignin extraction with enzymatic lignin depolymerization in biocompatible ionic liquids. A bi-enzyme system containing aryl alcohol oxidase (AAO) and lignin peroxidase (LiP) was developed to depolymerize lignin. Temperature and pH profiles for LiP and AAO were determined. Biocompatibilities of LiP and AAO in different deep eutectic solvents and ionic liquids were investigated. Aqueous cholinium glycinate was found to be an efficient and suitable solvent to solubilize lignin and serve as a biocompatible medium for enzymes to work. LiP and AAO together reduced lignin molecular weight in both solid and liquid phase after enzymatic lignin depolymerization.
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