Efficient removal of nitrate, manganese, and tetracycline in a novel loofah immobilized bioreactor: Performance, microbial diversity, and functional genes

The remediation of multiple pollutants in water, for instance, nitrate, heavy metals, and antibiotics is urgent and necessary for the global water resources protection. Herein, a modified loofah bioreactor was designed for simultaneous denitrification, manganese (Mn) oxidation, and tetracycline (TC) removal. The maximum removal efficiencies of NO3--N (91.97%), Mn(II) (71.25%), and TC (57.39%) were achieved at a hydraulic retention time (HRT) of 9 h, Mn(II) concentration of 20 mg L-1, and TC concentration of 1 mg L-1. SEM and XRD were carried out to characterize the bioprecipitation in the operation of bioreactor. TC addition affected the gaseous denitrification products, dissolved organic matter, as well as reduced the OTU in the bioreactor. The Zoogloea were regarded as the dominant species in the microbial community and played an essential role in the operation of bioreactor. Metagenomic analysis proved the great potential for denitrification, manganese oxidation, and antibiotic removal of loofah bioreactor.

Automated summary

A modified loofah bioreactor was designed for simultaneous denitrification, manganese oxidation, and antibiotic removal in water treatment. The reactor showed high removal efficiencies for nitrate, heavy metals, and antibiotics under specific conditions, offering great potential for global water resources protection. The study also identified key microbial species playing essential roles in the operation of the bioreactor.